Lipoprotein lipase (LPL) is a homodimeric enzyme required for the lipolytic processing of triglycerid-rich lipoproteins (chylomicrons and very low density lipoproteins) ( 1 – 3). Together, these data define a mechanism by which LPL mutations could elicit disease and provide insights into LPL sequences required for binding to GPIHBP1. Also, changing cLPL residues 421 to 425, 426 to 430, and 431 to 435 to alanine blocks cLPL binding to GPIHBP1 without inhibiting catalytic activity. In support of this idea, a chicken LPL (cLPL)–specific monoclonal antibody, xCAL 1–11 (epitope, cLPL amino acids 416–435), blocks cLPL binding to GPIHBP1 but not to heparin. These findings suggest that sequences downstream from LPL's principal heparin-binding domain (amino acids 403–407) are important for GPIHBP1 binding. Both mutations abolish LPL transport across endothelial cells by GPIHBP1. Here, we report that two LPL missense mutations initially identified in patients with chylomicronemia, C418Y and E421K, abolish LPL's ability to bind to GPIHBP1 without interfering with LPL catalytic activity or binding to heparin. Any such mutation would provide insights into LPL sequences required for GPIHBP1 binding. We hypothesized that some cases of chylomicronemia might be caused by LPL mutations that interfere with LPL's ability to bind to GPIHBP1. Earlier studies have established that chylomicronemia can be caused by LPL mutations that interfere with catalytic activity. An absence of GPIHBP1 prevents the entry of LPL into capillaries, blocking LPL-mediated triglyceride hydrolysis and leading to markedly elevated triglyceride levels in the plasma (i.e., chylomicronemia). □ Thank you so much for taking the time to view our products.Ĭopyright © 2021 BerryDesignShop, all templates are for personal use only and cannot be resold, shared, or used commercially without our written consent.GPIHBP1, a glycosylphosphatidylinositol-anchored protein of capillary endothelial cells, shuttles lipoprotein lipase (LPL) from subendothelial spaces to the capillary lumen. If you have any questions or issues, please feel free to send us a message! We do our best to reply to all messages within 24 hours. Your question not answered here? Send us a message and we’ll be more than happy to assist! Upload your own design, and proceed to follow the prompts and then print! You can get Avery to print for you and deliver, or you can print yourself from your home computer.ĭownload as a PDF and use the ‘Save Paper’ option to fit multiple templates onto the following page sizes:īleed & Trim Lines are available to assist with printing & cutting. This will generate the template necessary to ensure it matches 100% when printing.ĥ. Put in your Avery Software/Re-Order Number. This will allow you to save designs and access them again later.Ĥ. Go to the Avery Design & Print website: ģ. Once you've finished editing, download the finished template in JPEG format (make sure to delete the outline prior to downloading, as you will not need it for pre-cut paper).Ģ. Please ask any questions prior to purchase.ġ. We can not guarantee that these will match any other kinds of pre-cut paper. You can print these on a home computer and cut them out by hand, upload them to a printing house or use AVERY pre-cut paper (**please refer to instructions below on how to print with Avery paper**). There is no expiry on the template, so you can come back and edit & save it as many times as you’d like. Edit, Download & Print as many times as you’d like! The email is sent instantly once your payment has been processed, but in some cases it can take 10-15 minutes.ģ. You will then be sent an email from Templett with a direct LINK to your purchased template/s. This is a digital item, nothing physical is shipped from us.Ģ. This is a template that you are required to edit yourself. Need a different size? Send us a message to discuss a size change order! Trim & Bleed line options are available, to assist with printing and cutting. Use Templett’s ‘Save Paper’ option to print multiple designs per page when downloading as a PDF. JPEG: Professional Printing & Digital distribution PDF: Ideal for Home & Professional Printing ➡ Square Gift Tag without an outline - 2x2 inches ➡ Round Gift Tag with an outline - 2x2 inches This purchase includes the following editable templates: You then have the option to print from home or at a print shop of your choosing. After purchase, you’ll receive an email from Templett which gives you a direct link to your purchase. You can edit this gift tag directly from your web browser on your computer using ! All fonts & graphics are pre-loaded for you, so you don’t need to download anything (other than your beautifully finished template!).
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